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61 chemical substances were chosen from the updated suggested listings of genotoxic and non-genotoxic chemicals for assessment for the performance of brand new or enhanced genotoxicity examinations and tested in as much as five microbial strains. The arrangement utilizing the data through the clinical literary works (over 90%) confirms the dependability regarding the Ames MPF™ as a cost-effective and 3R-compliant alternative to the regulatory Ames test that enables to anticipate and assess chemical