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The authors suggest that the improved quality of the NIL may be due to the genomic segment carried along with the Lr24/Sr24 genes. The online version contains supplementary material available at 10.1007/s13205-021-02679-x. The online version contains supplementary material available at 10.1007/s13205-021-02679-x.Konjac glucomannan oligosaccharide has attracted much attention due to its broad biological activities. Specific glucomannan degrading enzymes are effective tools for the production of oligosaccharides from konjac glucomannan. Ho