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Our present result suggests that β-sitosterol mediated AgNP induce apoptosis in colon cancer cells and this finding may pave the way for further experimental analysis in vivo. The purpose of this study was to systematically review the prevalence of class 1 integrons, antibiotic resistance pattern in isolated from clinical samples other than burn samples. The Web of Science, PubMed, Scopus, and Science Direct databases were searched using keywords based on the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) gui