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On the basis of EC50, the ecological risk assessment was further carried out, and HC5 value of nonylphenol and 1, 2-dichloroethane to aquatic organism was 0.079 and 44 mg L-1, respectively.In the study, we assessed how acute changes in water temperature and dissolved ammonia concentration can affect the swimming behavior pattern of rainbow trout (Oncorhynchus mykiss). The behavior was analyzed in three different stages 1) increase stage, (where temperature and ammonia concentration increase during this step, resp