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The isolated ginsenoside Rb can be used in further pharmaceutical studies. Herein, we demonstrated a novel enzymatic elimination-combined purification method for the chromatographic purification of ginsenoside Rb . This method can also be applied to purify other isomeric glycoconjugates in mixtures. Herein, we demonstrated a novel enzymatic elimination-combined purification method for the chromatographic purification of ginsenoside Rb3. This method can also be applied to purify other isomeric glycoconjugates in mixtures. The reports about valuable oligosacc