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Nevertheless, little is known about the regulatory system of flavonol biosynthesis in Tartary buckwheat. In this research, we identified and characterized a novel SG7 R2R3-MYB transcription consider Tartary buckwheat, FtMYB6. We showed that FtMYB6 is located within the nucleus and will act as a transcriptional activator. The FtMYB6 promoter showed strong spatiotemporal specificity and ended up being caused by light. The phrase of FtMYB6 showed a significant correlation with rutin buildup in the origins, stems, leaves, and blossoms