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For much better evaluating result, the focus of 5-LOX enzyme, incubation temperature and time, pH and ion power were enhanced. In inclusion, 5-LOX inhibitory assay in vitro and molecular docking strategy were utilized for further confirmation. OUTCOMES 20 substances were characterized when you look at the ultrafiltrate by high definition Fourier change ion cyclotron resonance size spectrometry (FT-ICR MS) and 16 ligands revealed binding capacity to 5-LOX. Included in this, six ligands were dedu