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The phrase of defense-related genes, including FRK1, PR1, WRKY22, and WRKY29, were less induced in RTNLB4 overexpression (O/E) transgenic plants after A. tumefaciens elf18 peptide treatment. Pretreatment with elf18 peptide decreased Agrobacterium-mediated transient expression effectiveness more in wild-type seedlings than RTNLB4 O/E transgenic flowers, which implies that the induced defense responses in RTNLB4 O/E transgenic flowers could be affected after bacterial elicitor treatm