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This paper aimed at to evaluate the qualitative effects of climatic seasonality in a subtropical lagoon, using Discriminant Analysis, as well to identify the most sensitive and responsible parameters for these changes. The Mirim Lagoon watershed is one of the main transboundary basins in South America and is of great economic importance for the region because its waters are used for irrigation of rice fields and for the potable water supply to populations close to it, in this way, these uses may affect the quality of the basin waters. T