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As a result, a 31P NMR study and a kinetics study strongly supported not only the acid-base cooperative mechanism as previously proposed by other researchers but also the mixed anhydride mechanism as presently proposed by us.A key event in the ATP-driven transport cycle of the calcium pump sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) occurs when autophosphorylation of the pump with two bound ions Ca2+ triggers a large conformational change that opens a gate on the luminal side of the membrane allowing the release of the ions. It is b
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