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The intraperitoneal injection of NDEA (35 mg/kg) began at 4 weeks of age and was carried out at regular intervals thereafter. Whilst the fatality rate ended up being significant when you look at the KO mice, AsA supplementation (1.5 mg/ml in the normal water) greatly longer their life-spans. Just two away from 54 KO mice survived to 28 days, and both contained about an order of magnitude greater range cyst nodules when compared with WT mice or KO mice with AsA supplementation.