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Thus, contamination of well water by this carbapenem-resistant and presumptively virulent strain of R. ornithinolytica should be considered a potential public health risk. Pathways for the physical disintegration of biochar (BC) and the release of water dispersible BC colloids (WDBC) have received much attention due to their unique impacts on carbon loss and contaminant. However, the current understanding of the mechanisms involved in WDBC formation and associated influencing factors is rather limited. This study systematically explore