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The establishment of a germ cell culture contributes to efficient SSC propagation in rare, endangered, or commercially cultured fish species for use in biotechnological manipulation, such as cryopreservation and transplantation. Finally, we discuss organ culture and three-dimensional models for in vitro investigation of fish spermatogenesis.Simultaneous production of hydrogen and degradation of cyanide ion and methyl red dye were successfully accomplished by employing nano-particles of TiO₂-CuO under the radiation of UV li