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The incorporation of crop straw with fertilization is beneficial for soil carbon sequestration and cropland fertility improvement. Yet, relatively little is known about how fertilization regulates the emissions of the greenhouse gas nitrous oxide (N2O) in response to straw incorporation, particularly in soils subjected to long-term fertilization regimes. Herein, the arable soil subjected to a 31-year history of five inorganic or organic fertilizer regimes (unfertilized; chemical fertilizer application, NPK; 200% NPK application, 2 × NP