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The purpose of the study was to explore the possible deleterious effects of Methotrexate (MTX) treatment on the urogenital tissues and the potential protective effects of Astaxanthin (AXA). Twenty-four female Wistar Albino rats (12months old) were divided into 3 groups as follows Group I (Control group) rats received a single dose of 0.1ml saline by gavage and intraperitoneal injection. Group II (MTX group) rats received a single dose of 20mg/kg MTX, i.p, on the 2nd day. Group III (MTX + AXA group) rats received 100mg/kg AXA orally for 7
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