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To examine and contrast the levels of perceived non-specific and work-related stress and sources of work-related stress between medical and psychiatric nurses. The comparative cross-sectional study was conducted at one psychiatric and three general hospitals in Lahore, Pakistan, from November 2014 to February 2015. Data was collected using a self-report survey administered to psychiatric and medical nurses. Instruments used to collect data comprised a demographic sheet, the perceived stress scale and the Devilliers, Carson and Leary stres