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The conducted analyses suggest that another major gene or other factors were influencing kernel texture. The online version contains supplementary material available at 10.1007/s13205-021-02897-3. The online version contains supplementary material available at 10.1007/s13205-021-02897-3.The present study was aimed at producing enhanced and sustained bioelectricity from distillery wastewater in a double chamber microbial fuel cell (MFC) by changing inter-electrode distance, inoculum and reactor volume. Using double chamber MFC with 1 L working volume, when t