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Functional enrichment analysis suggested that the metabolic path, a reaction to stimulus and catalytic task were notably enriched under stronger freezing stress. Among the list of DEGs, nine participated in the Ca2+ signal pathway, thirty-two were identified to participate in sucrose metabolism, and others had been identified to participate in the legislation of ROS, plant hormones and antifreeze proteins. In addition, important transcription facets (WRKY, AP2/ERF, NAC and bZIP) participating in freezin