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ignaling pathways of the cell cycle, ubiquitin-mediated proteolysis, mismatch repair and p53 were enriched in the GNPNAT1 overexpression group. GNPNAT1 may be a potential prognostic biomarker and novel target for intervention in LUAD. GNPNAT1 may be a potential prognostic biomarker and novel target for intervention in LUAD.Epigenetics is an essential biological frontier linking genetics to the environment, where DNA methylation is one of the most studied epigenetic events. In recent years, through the epigenome-wide association study (E