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Lanthanide-doped upconversion nanoparticles (UCNPs) have attracted great attention in bioimaging applications. However, the stability and resolution of bioimaging based on UCNPs should be further improved. Herein, we synthesized SiO2-coated Ga(III)-doped ZnO (GZO) with lanthanide ion Yb(III) and Tm(III) (Yb/Tm/GZO@SiO2) UCNPs, which realized red fluorescence imaging in heart tissue. With increasing injection concentrations of Yb/Tm/GZO@SiO2 (1-10 mg/kg), the red fluorescence imaging intensity of heart tissue gradually increased. Moreove