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ofessionals in their clinical decision making and highlight areas for future research in this important clinical area. Open Science Framework submitted on 9 July 2020. https//osf.io/mpksq/ . Open Science Framework submitted on 9 July 2020. https//osf.io/mpksq/ . Isoprenol is the basis for industrial flavor and vitamin synthesis and also a promising biofuel. Biotechnological production of isoprenol with E. coli is currently limited by the high toxicity of the final product. Adaptive laboratory evolution (ALE) is a promising method to add