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Methionine is one of the many amino acids in the soil. In order to study the role of methionine in acidic forest soil, the effect of methionine (Met) was compared with control together with addition of ammonium (Met + A), nitrite (Met + N), and glucose (Met + C) under 60% or 90% water holding capacity (WHC), because ammonium and nitrite are important factors in nitrification, and glucose affect the heterotrophic nitrification and nitrogen immobilization. We found that methionine addition significantly reduced NO3- concentration in acid