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Increased focus on the development and application of bioavailability-based metal water quality guideline values requires increased understanding of the influence of water chemistry on metal bioavailability and toxicity. Development of empirical models, such as multiple linear regression models, requires the assessment of the influence of individual water quality parameters as toxicity modifying factors. The present study investigated the effect of pH on the lability and toxicity of zinc to a tropical green microalga (Chlorella sp.). Z