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This shift was likely accompanied with adaptation to a narrow tree line habitat and switch to a pioneer decomposer strategy, both requiring rapid colonization of a nitrogen-limited substrate. Among substrate specialists with narrow niches, we also found evidence for pathways facilitating reversal to generalism, highlighting how evolution may move along different axes of niche space.Non-homologous DNA end joining (NHEJ) is the predominant repair mechanism of any type of DNA double-strand break (DS during most of the cell cycle and is