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Fish had been challenged aided by the various bacterial isolates both by immersion and intramuscular injection. Various levels of bacteria were tested to gauge their particular pathogenicity. Injection with 1 × 105 CFU of A. hydrophila and A. sobria resulted in clinical signs exactly the same as those of purple spot-diseased grass carp in Vietnam. Nothing associated with various other bacterial isolates tested triggered any morbidity or death in seafood challenged eit