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05), but MT ammonia-N treatment significantly decreased than ammonia-N group (p less then 0.05). Compared with the control group, ammonia-N significantly reduced the activities of Trypsin in the intestine and hepatopancreas (p less then 0.05), while MT ammonia-N group can significantly improve the intestinal trypsin activity than ammonia-N (p less then 0.05). The intestinal microbiota of E. sinensis results showed that ammonia-N stress significantly decreased the relative abundance of Bacteroidetes (p less then 0.05). Ammonia-N stress