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Mutations in human MSX1 are associated with orofacial clefting and we show here that Msx1 deficiency causes a rise defect of the medial nasal procedure (Mnp) in mouse embryos. Although this defect alone doesn't interrupt lip development, Msx1-deficient embryos develop a cleft lip as soon as the mother is transiently exposed to reduced air amounts or even phenytoin, a drug recognized to trigger embryonic hypoxia. When you look at the absence of socializing environmental factors, the Mnp g