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The aim of this study was to investigate the effect of iron or/and zinc supplementation and termination of this treatment on the antioxidant defence of the male reproductive system and sperm viability in rats. The study consisted of 3 stages I) 4-week adaptation to the diets (C-control or D-iron deficient); II) 4-week iron and/or zinc supplementation (10-times more than in the C diet of iron CSFe, DSFe; zinc CSZn, DSZn; or iron and zinc CSFeZn, DSFeZn; and III) 2-week post-supplementation period (the same diets as during stage I). Param