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Total serotype-specific antibody titers also neutralizing titers determined from pseudovirus infectivity assays had been unaffected by incubation regarding the ALD-coated vaccines for at 4, 50, or 70 °C for 90 days just before administration. In inclusion, even after incubation for 90 days at 70 °C, single doses of ALD-coated vaccines produced both higher total antibody answers and greater neutralizing reactions than control immunizations that used two doses of
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