https://dna-metabolism.com/pro....gnostic-analysis-ass
Right here, we develop RNA linkers to mix theophylline- and 3-methylxanthine (3MX)-binding aptamers aided by the sgRNA, enabling small molecule-dependent editing in Escherichia coli. These activatable guide RNAs enable temporal and post-transcriptional control of in vivo gene editing. More, they reduce the death of host cells due to slices within the genome, a significant restriction of CRISPR-mediated bacterial recombineering.Petroc