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An immunoassay was developed that utilized plasmonic coupling between immobilised gold nanorods and colloid gold nanospheres to detect the marine toxin domoic acid (DA). The aspect ratio of the nanorods was optimised and the effects of variation in acidity, silver to gold ratio, cetyltrimethylammonium bromide (CTA concentration and seed addition in the growth solution on the yield, size variance and LSPR peak position was investigated. Excellent nanorods (size variation less then 15%; aspect ratio 3.5-5; yield 0.26-0.35 nM mL-1) were
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