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The probe Mito-Phos is highly biocompatible with the ability to assess ALP expression in mitochondria in live cells.UV light-emitting diodes (UV LEDs) are an emerging technology and a UV source for pathogen inactivation, however low UV-LED wavelengths are costly and have low fluence rate. Our results suggest that the sensitivity of human Coronavirus (HCoV-OC43 used as SARS-CoV-2 surrogate) was wavelength dependent with 267 nm ~ 279 nm 286 nm 297 nm. Other viruses showed similar results, suggesting UV LED with peak emission at ~286 nm could serve a