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All of the above results would be helpful to understand the tolerance mechanisms of alfalfa reproductive organs to both B deficiency and B surplus conditions, and also to give insight into the regulatory role of B in improving seed yield and germinability in alfalfa seed production. In summary, B likely plays a structural and regulatory role in relation to lipid metabolism, carbohydrate metabolism, amino acid metabolism, and signal transduction, thus regulates alfalfa reproductive processes eventually affecting the seed yield and germinability of alfalfa se