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OBJECTIVES Multidrug-resistant (MDR) enterococci were found extensively in the food samples. This study characterized the phenotypic virulence factors and the ability of horizontal gene transfer of a streptomycin resistance gene among enterococci isolated from fermented pork. METHODS Thirty-six MDR enterococci were subjected to screening of gelatinase, biofilm formation at various temperatures (4, 25 and 37 °C), clumping ability and conjugation. RESULTS All positive-gelatinase and positive-clumping strains wereEnterococcus faecalis (41.7