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Numerous systematic conclusions have introduced that long noncoding RNAs (lncRNAs) may be involved in cyst occurrence and development. Although DSCAM-AS1 has been studied in a number of cancers, its part and mechanism in OS are poorly recognized. In this work, advanced level of DSCAM-AS1 had been validated in OS cellular outlines. Depleting DSCAM-AS1 inhibited cell proliferation, migration and EMT process in OS. Subsequently, we disclosed that DSCAM-AS1 had been mainly observed in the cytoplasm of OS cells and might bind with