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Soil nematode communities play an important role in ecosystem material cycling and energy flow. In this study, soil samples were collected from three rotation systems in southern Ningxia mountainous region, including alfalfa continuous cropping (A-A), alfalfa-corn rotation (A-C), alfalfa-potato rotation (A-P). Soil physicochemical properties, nematode community composition and their metabolic footprints were measured. Compared with the A-A plot, the concentrations of soil organic carbon (SOC) and total nitrogen (TN) were significantly inc