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Compared with the phosphorus forms of the Tiaoxi watershed, NaOH-P contents were significantly higher compared to HCl-P, which was significantly higher in the Hongze watershed. In comparison, NH4Cl-P contents were significantly lower in both. Variations may be attributed to differential discharge of the P form in the watershed due to land-use changes and urban river ambient conditions.Classical synthetic protocols were applied for the isolation of three novel 1,3,4-thiadiazole derivatives which were then complexed with the biologically i