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S126 has never been considered to play a role in the enzyme's activity or stability, thus the finding showed the importance of this residue.Temperature limits the geographic ranges of several tick species. Little is known about the thermal characteristics of these pests outside of a few studies on survival related to thermal tolerance. In this study, thermal tolerance limits, thermal preference, and the impact of temperature on activity levels and metabolic rate were examined in larvae for six species of ixodid ticks. Tolerance of low t