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To analyze biofouling minimization methods, studies have a tendency to give attention to single-species biofilms; selection of system is essential to making useful outcomes. To determine a best-practice system for studying antimicrobial treatment of biofilms, with certain curiosity about biofouling of RO membranes, we responded listed here two questions, each via its semi-systematic review 1. Which organisms can be utilized to try antimicrobial efficacy against biofilms on RO me