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To present the first robotic-assisted uterus retrieval from a living donor for uterine transplantation in Brazil. Stepwise demonstration of the technique with narrated video footage. A uterine transplantation was performed in a 33-year-old patient with Mayer-Rokitansky-Küster-Hauser syndrome. The donor was a 50-year-old woman who underwent a robotic-assisted uterus retrieval. The procedure was held at Barretos Câncer Hospital, Barretos - São Paulo, Brazil. After approval from the National and local Institutional Review Board, the protocol w