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Published by BMJ.Lactococcus lactis encounters various environmental challenges, especially acid stress, during its growth. Cell wall can maintain the integrity and shape of the cell under the environmental stress, and D-amino acids play an important role in the cell wall synthesis. Here, by analyzing the effects of 19 diverse D-amino acids on the physiology of L. lactis F44, we found that the exogenously supplied D-methionine and D-phenylalanine could increase the nisin yield by 93.22 % and 101.29 % respectively as well as significantly i