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The results of this study will fully understand the metabolic patterns of bacteria under alkaline conditions, expand the range of available functional bacteria, and enhance the practical aspects of co-contaminants remediation. A multi-activity three-dimensional quantitative structure-activity relationship (3D-QSAR) model was established based on the comprehensive evaluation index (CEI) of polychlorinated naphthalenes (PCNs). The CEI values were calculated using the vector analysis method in combination with the following parameters biolog