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Viral DNA was extracted from prepared examples using a Qiagen viral DNA extraction Kit (QIAamp DNA small Kit). To detect four typical risky HPV types (16, 18, 31, 45), multiplex real-time polymerase chain response (PCR) concentrating on the LCR/E6/E7 region associated with the HPV genome had been performed in ABI 7500 cycler (Applied Biosystems). The bad samples were screened by conventional PCR targeting the L1 capsid region to