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BACKGROUND AIM Several studies observed alterations in the gut microbiota in patients with non-alcoholic fatty liver disease (NAFLD). However, analyzed patient populations and methods strongly differ among these studies. The aim of this study was to prove the reproducibility of published results and to provide a detailed overview of all findings in our NAFLD cohort using next generation sequencing methods. METHODS The individual taxonomic microbiota composition of fecal samples from 90 NAFLD patients and 21 healthy controls was an