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Western blot revealed that Ad-EC could inhibit the activation of AKT pathway and ERK1/2 pathway, while Cetuximab had the AKT pathway over-activated. In vivo experiments with HEP-2 xenograft in nude mice confirmed the tumor inhibition in Ad-EC, CDDP and Ad-EC plus CDDP groups compared with PBS group (P less then 0.01). Collectively, these data support the effective inhibition of cancer cells by this novel gene therapy strategy.Hepatocystis is a genus of single-celled parasites infecting, amongst other hosts, monkeys, bats and squirrels. A