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gainst microbial infections, particularly QDs500 . Heel sticks account for most blood tests performed in neonates without analgesia because topical local anesthetics are ineffective on heel glabrous skin. We investigated the antinociceptive effect of an alternative topical analgesic, a vapocoolant spray, on hind paw glabrous skin of rat pups. The spray was applied by two methods method 1 for 4s at a distance of 8cm and method 2 for 10s at a distance of 18cm. The rat pups were randomized to either method 1 (n=32) or method 2 (n=31). Vapoc
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