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At this time point, 2-mercaptoethanol in the HgCl2 + 2-mercaptoethanol group reversed the effects of HgCl2 as compared to the HgCl2 group. Present study showed the expression and immunolocalisation of AQP3 in human spermatozoa and the potential role of AQPs in the sperm motility and MMP. © 2020 Blackwell Verlag GmbH.AIMS Immune checkpoint inhibitors (ICIs) have revolutionized the treatment of advanced malignancies by boosting immune-mediated destruction of neoplastic cells, but course with side effects stemming from generalized immune s