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The floral morphology of Cymbidium ensifolium, a well-known orchid in China, has increasingly attracted horticultural and commercial attention. However, the molecular mechanisms that regulate flower development defects in C. ensifolium mutants are poorly understood. In this work, we examined a domesticated variety of C. ensifolium named 'CuiYuMuDan', or leaf-like flower mutant, which lacks typical characteristics of orchid floral organs but continues to produce sepal-to leaf-like structures along the inflorescence. We used comparative