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The samples were kept in cell conservation method containing real human albumin, Iscove's and vancomycin preserved at 4°C. The focus of proteoglycans within the extracellular method had been quantified by way of Safranin-O, while tissue structural analysis had been considered by histological research with hematoxylin-eosin stained slides. The pictures gotten were analyzed in line with the histological scores of Mankin while the score recommended by the OsteoArthritis analysis Society Overs