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The conjugation regarding the hydrophilic polymer resulted in the amphiphilic character regarding the conjugates, which resulted in self-aggregation and nanoparticle formation that reduced the cellular uptake above a certain aggregation focus. Having said that, the hydrodynamic volume therefore the various polymer sequence topology of the linear PEG as well as the compact hyperbranched HbPG additionally played an important role within the biological activity. Therefore, in similar m